As expected, subjects with HCC or T2DM + HCC had significantly higher gamma-glutamyl transferase (r-GT), alkaline phosphatase (ALP), alpha-fetoprotein (AFP), total bilirubin (TBIL), and direct bilirubin (DBIL) than those in the healthy population group, indicating the liver function damage. The gene discussed is AFP; the disease is type 2 diabetes mellitus.