GCG and type 2 diabetes mellitus: Glucagon stimulation produced no significant changes in VAT glucose or pyruvate consumption, with the sole exception of the Ob + Pre-T2D group, in which glucagon stimulation (10 nM) increased VAT pyruvate consumption (Ob + Pre-T2D: glucagon (0 nM), 2.20 ± 0.04 nmol/mg of VAT vs. glucagon (10 nM), 2.44 ± 0.11 nmol/mg of VAT, Δ 10.91%, p < 0.05) (Figure 4, Table S3).