In VAT from Ob + Pre-T2D, GLP-1 (100 nM) stimulation decreased isoleucine consumption (Ob + Pre-T2D: GLP-1 (0 nM), 0.84 ± 0.06 nmol/mg of VAT vs. GLP-1 (100 nM), 0.00 ± 0.23 nmol/mg of VAT, Δ −99.45%, p < 0.05), and it increased pyroglutamate consumption (Ob + Pre-T2D: GLP-1 (0 nM), 12.28 ± 15.0.59 nmol/mg of VAT vs. GLP-1 (1 nM), 13.11 ± 0.81 nmol/mg of VAT, Δ 6.75%, p < 0.01). Here, GLP1R is linked to type 2 diabetes mellitus.