The cells in seqC1_FoxP3int had a relatively low expression of FoxP3, CD25, CTLA-4, HLA-DR, PD-1 and IL-10, and a high expression of IL-17, indicating that these may be pro-inflammatory Tregs induced in an inflammatory microenvironment in these tumor and lymph node tissues [35,36]. This evidence concerns the gene CTLA4 and neoplasm.