The largest cluster was identified predominantly in tumors and lymph nodes, with only a small component in the blood, and was consistent with previous reports from NSCLC tumors with a high expression of FoxP3, CD25, CTLA-4, HLA-DR and IL-10, and low expression of Helios [4,38,41,42]. The gene discussed is IKZF2; the disease is non-small cell lung carcinoma.