Taken together these findings, our study demonstrates the fundamental role of OLIG2 and MYT1L transcription factors in hUC-MSCs differentiation toward oligodendrocyte-like cells, which were greatly facilitated by the oligo induction medium, emphasizing the significance of transcriptional regulators as a remarkable tool for stimulating myelin repair in lethal demyelinating disorders. Here, MYT1L is linked to demyelinating disease.