For example, a previous study found that the infiltration levels of intraepithelial FoxP3+ cells in poor or undifferentiated carcinomas were higher.17 In gastric cancer, high infiltration of CD163+ TAMs and CD66b+ TANs are significantly related to poor differentiation and well differentiation, respectively.18 However, we did not found studies that systematically and simultaneously explored the effects of the degree of tumor cell differentiation on infiltration of FoxP3+ Tregs, CD66b+ TANs, or CD163+ TAMs in the tumor microenvironment of CRC. This evidence concerns the gene CEACAM8 and carcinoma.