Although the lack of insulin response in quadriceps might seem paradoxical, most studies on GLUT4 translocation in muscle use high insulin doses (about 2 IU/kg), which are 10 times higher than the insulin doses used for the treatment of T2D patients and the dose used for this study (0.2 IU/kg) (2, 21, 51). Here, INS is linked to type 2 diabetes mellitus.