RT-qPCR showed that the OGT/OGA ratio increased in symNs cultured in the diabetes-mimicking environment (5–30 mM transition of glucose, as established in Figure 1), even compared to the regular consistent 25 mM glucose condition (Figure 5A), suggesting that O-GlcNAcylation levels may be elevated in this model. The gene discussed is OGT; the disease is diabetes mellitus.