We confirmed lack of a large effect with S. Typhimurium by infecting susceptible C57BL/6J mice with Mcoln2 knocked out31 via intraperitoneal injection—which avoids restriction by stomach acid or variance introduced by gut microbiota—and quantified S. Typhimurium burden in the spleen 4 days post infection (Figure 2I). The gene discussed is MCOLN2; the disease is infection.