In the current study, we assessed the therapeutic effect of Mg2+ supplement and insulin on T2DM-induced inflammatory responses and tissue injury in the rat gastric antrum by assessing changes in trypsin-1, PAR receptors, PI3K/Akt/p-Akt, and COX-2 proteins levels and the histopathological index. The gene discussed is PRSS1; the disease is type 2 diabetes mellitus.