Moreover, upon antigenic activation in vitro, PB CAR-T cells released lower levels of IFN-γ, IL-6, IL-10, TNF-α, CCL2, CXCL10, and GM-CSF than Lenti CAR-T cells, but demonstrated a robust release of IL-9, indicative of a distinct anti-tumor response pathway. This evidence concerns the gene TNF and neoplasm.