Statistical analysis on day 6 of co-culture with CD8+ T cells showed a significant reduction of the tumor sphere area under the combination treatment with IL-12 compared to the same treatment without IL-12 in all cell lines (G9_pCDH, p = 0.0008**** –0.0728 ns, n = 3) under co-culture with IL-12 (Figure 4D). This evidence concerns the gene CD8A and neoplasm.