LaminA/C, a marker for nuclear protein, was not detected in the cytoplasmic fraction, and β-tubulin, a marker for cytoplasmic protein, was not detected in the nuclear fractions, suggesting that neither fraction is contaminated by the counterpart (Fig. 4), validating the findings that virus infection promotes depletion of cytosol NFAT5 but differentially influenced accumulation of nuclear NFAT5 of different isoforms. The gene discussed is NFAT5; the disease is viral infectious disease.