After analysis of the overlap of biomarkers obtained using the two algorithms, 20 candidate biomarkers were finally obtained (Fig. 5D and Supplementary Table S8), as follows: AAK1, AIFM1, CCDC114, PTPRN2, TMEM54, CDH15, CXCR1, ABI3BP, FAM149A, SLC9A3R1, ISPD, VIT, TEAD3, BAK1, PSMD3, FUT11, PLCG2, CFP, TRAPPC6B, and JDP2. Most genes were upregulated in PCOS, except AAK1, CCDC114, PTPRN2, CDH15, ISPD, FUT11, and CFP (Fig. 6A and B). This evidence concerns the gene CFP and polycystic ovary syndrome.