Next, we prepared paraffin-embedded prostates of 6-month-old PtenHNP/HNP (HNP/HNP), Pten+/–, and Pten+/+ mice and serially sectioned these, collecting 5 μm sections for H&E staining and PIN lesion quantitation every 200 μm. Here, PTEN is linked to prostate intraepithelial neoplasia.