We observed that the SOD3 recombinant protein significantly altered the expression of 2 genes in liver HepG2 cells, most importantly RNF128. RNF128 is a transcription factor, the overexpression of which has previously been shown to enhance hepatic lipid accumulation and increase the expression of lipid metabolic genes in mice and liver cells,16 and RNF128 knockout mice were also found to be resistant to developing liver steatosis on a high-fat diet compared to control mice, indicating that its absence plays a protective role against NAFLD.16 This evidence concerns the gene SOD3 and metabolic dysfunction-associated steatotic liver disease.