To select IVs for the reverse direction MR analysis, we first selected liver aware DE genes, which were DE in the liver but not in the adipose tissue (n = 304 genes), selected those with a significant liver cis-eQTL and a NAFLD GWAS hit that was not also a TG GWAS hit in their cis regions (n = 1 valid cis region), and tested those for colocalization in the single remaining cis region of the NEDD4 Like E3 Ubiquitin Protein Ligase (NEDD4L) gene (see Methods). Here, UBE2E3 is linked to metabolic dysfunction-associated steatotic liver disease.