We first investigated DC maturation in lymph nodes around subcutaneous 4T1 tumors in vivo after day 3 following the schedule in Fig. 4B. Levels of CD80 and CD86 of DCs in the LEH-CSPC + laser group showed enhancements compared with those of LEH-CSPC alone, indicating the importance of the PDT effect on antigen release from tumor cells in vivo and subsequent DC maturation. This evidence concerns the gene CD86 and neoplasm.