SMAD4, MUC16, BRCA2, KRAS, ERBB2, NF1, TERT, and MDM2 were found to be associated with OS of CCA in the univariable Cox analysis, in those MUC16, ERBB2, and MDM2 did not show significant association with OS in the multivariable Cox analysis. This evidence concerns the gene NF1 and cholangiocarcinoma.