Furthermore, the immunohistochemistry results revealed that the expressions of HMGB1, p65, the pyroptotic proteins NLRP3 and cleaved caspase-1, and the senescence-related protein p21 were increased in the IDD group and markedly decreased in the rosuvastatin group compared with those in the IDD group (Figure 7E‒I). This evidence concerns the gene HMGB1 and intervertebral disk degenerative disorder.