Among the samples studied here, four tumors from three patients were classified as diffuse midline glioma K27 mutant (P2233_104T, P1148_113T, P7708_127T, P7708_128T) and harbored mutations in H3F3A in addition to TP53 or ATRX mutations. The gene discussed is TP53; the disease is diffuse midline glioma.