Our trajectory analysis of CD8 + T cells found that most of the CD8 + T cells in metastasis were located in the middle and posterior segment of the whole cell differentiation, and CD8 + T cells showed a high expression of cytotoxic markers GZMM and GZMB (Supplementary Fig. 4, Supplementary Material 15), implying that GZMK + subsets in PCa were intermediate states in the CD8 + T cell transition process. This evidence concerns the gene GZMM and posterior cortical atrophy.