Our multicolor immunofluorescence staining on paraffin sections of 13 cases of PCa combined with lymphatic metastasis indicated that CXCR4 was positive in the primary tumor tissue and lymphatic metastases, and the positive rate was higher in the metastatic lesions than in the primary lesions, which was consistent with the results of single cell sequencing. Here, CXCR4 is linked to neoplasm.