We can conclude that the patients who get pregnant at last have a younger age (31.06 ± 4.45 vs 33.29 ± 5.66, P < 0.001), shorter years of infertility (3.48 ± 2.38 vs 4.27 ± 3.76, P < 0.001), less Gn amount (2471.97 ± 1005.02 vs 2532.89 ± 1188.95, P = 0.01), and lower E2 (2399.08 ± 2144.44 vs 2527.66 ± 2416.90, P = 0.02) as well as LH (2.66 ± 3.60 vs 3.88 ± 6.10, P < 0.001) levels on trigger day compared with those who do not in the first cycle. The gene discussed is PLOD1; the disease is Infertility.