To verify that DCM-associated mutations in other domains/regions outside the RS domain do not impair nuclear localization of the protein, we engineered the G40W (20), L83I (5), S415N, V535I (4), E913K (9), and R1182H (8, 20) mutations into the respective domains/regions of human RBM20, and the localization of these constructs was determined by ICC in transfected H9c2 cells (Figure 5C). Here, RBM20 is linked to familial dilated cardiomyopathy.