NOS3 and atherosclerosis: While many immune pathways were enriched in the upregulated DEGs (e.g., CXCR5, IL21R, CCR7 and CD22) (A,C), pathways such as “lipid and atherosclerosis’ and “fluid shear stress and atherosclerosis” were enriched in the downregulated DEGs (such as NOS3, KLF2 and KLF4) (Figure 4B–D).