NUAK1 and melanoma: Using the relative expression of neonatal NHEMs in PMA-containing media and specific melanoma cell lines (A375 and SK-MEL-28) in high tyrosine media as benchmarks common to multiple studies, we assessed the effect of disparate miR-211 levels on validated target mRNA (NUAK1/ARK5, IGFBP5, PRAME, TGF-βRII, SLUG, and CHD5) measured by RT-qPCR [109,122,127].