As expected, absolute numbers of CD3+ TILs the relative proportion of CD4+IFN-γ+ cells among CD3+ TILs, and CD8+ granzyme B+(GzmB) cells were substantially increased in Lv-ISG15 tumor tissues (Fig. 2G) (The gating strategy is supplemented in Additional file 7: S7). The gene discussed is GZMB; the disease is neoplasm.