Furthermore, conditioned medium (CM) of CAF educated MDSCs increased the level and activity of 5-LO in MDSCs, thereby enhancing the stemness of MDSCs, and determined that CAF have an important influence on the formation of ICC stemness by mediating the overactivated 5-LO/LTB4-BLT2 metabolism in MDSCs [99]. This evidence concerns the gene ALOX5 and intrahepatic cholangiocarcinoma.