TLR-4 protein levels were significantly decreased when NAC was administered to pregnant dams only with subsequent NEC conditions (NAC-NEC) compared to both in the brain (1.53 ± 0.41 vs. 2.5 ± 0.6 U, p < 0.05) and ileum (0.12 ± 0.03 vs. 0.24 ± 0.04 U, p < 0.05). The gene discussed is TLR4; the disease is necrotizing enterocolitis.