Regarding differences in the degree of VAT inflammation associated with obesity, flow cytometry revealed significantly higher number of CD45+ Lin− CD11b+ Ly6Chigh CXCR1+ MHCII− inflammatory monocytes (Fig. 4A,B) and CD45+ Lin− CD11b− CD11c+ MHCII+ monocytes (Fig. 4C,D) in VAT from End.LepR-KO mice compared to End.LepR-WT controls. This evidence concerns the gene CXCR1 and obesity due to melanocortin 4 receptor deficiency.