LEPR and obesity due to melanocortin 4 receptor deficiency: The experimental work flow to examine the role of endothelial leptin receptors for diet-induced obesity in male and female End.LepR-WT and End.LepR-KO mice is shown in Fig. 2A. In mice fed SD, mean body weights did not significantly differ between End.LepR-WT and End.LepR-KO mice (males: 28.4 ± 0.4 vs. 28.1 ± 0.3 g, P = 0.5623; females: 21.1 ± 0.3 vs. 21.6 ± 0.2 g, P = 0.1210).