As shown in Fig. 1D, the tongue squamous cell carcinoma cell lines SCC4, SCC9, SCC15, and SCC25 do not express the CDK2AP1 protein, whereas the OSCC cell lines CA1 (derived from floor of the mouth), LM (mandibular region of the mouth), LUC4 (floor of the mouth), and two non-cancerous immortalized control cell lines, namely OKF-6 (normal human oral keratinocyte) and HEK293T (human embryonic kidney), are positive for the 12 KDa protein (Fig. 1D). Here, CDK2AP1 is linked to tongue squamous cell carcinoma.