This result confirmed that miR-205 down-regulated genes involved in the regulation of actin cytoskeleton upon the induction in HF-SCs in vivo, including Actb, Actn1, Actn4, Ctnna1, Rock2, and Piezo1, identified by the direct ligation of miR-205 and targeted sites (Fig. 3D and Dataset S1). The gene discussed is ACTN4; the disease is hydrops fetalis.