During co-staining NeuN (labeled for neurons) or CD31(labeled for ECs) with TRPM2, it showed the enrichment of TRPM2 protein expression in both neurons and ECs, moreover, the expression of TRPM2 protein in ECs was significantly stronger than that in neurons in spinal cord from T1D+SCI group (Figure S 2F and Figure 5H). This evidence concerns the gene PECAM1 and type 1 diabetes mellitus.