Furthermore, and to validate the role of PARP-1 in melanoma radioresistance, we performed a specific knock-out (KO) of the PARP-1 gene in the radioresistant HBL cells (Figure 1E) that caused a pronounced decrease in cell survival after exposure to RT compared to HBL SCR (Figure 1F), supporting the functional role of PARP-1 in melanoma radioresistance. Here, PARP1 is linked to melanoma.