In the present research, our findings further confirmed that, in terms of malignant phenotypes, miR-93-5p interference successfully replicated the inhibiting effect of HNRNPA2B1 knockout, and transfection of miR-93-5p mimics partially rescued the effect of HNRNPA2B1 silencing in prostate cancer cell lines. The gene discussed is HNRNPA2B1; the disease is prostate cancer.