Additionally, we stained PD1 and PD-L1-vital checkpoint protein in immune escape and found relatively higher tumorous infiltration of PD1+/CD3+ cells ratio in HL-related ICC patients which indicates PD1 blockade that reverse exhausted status and then restore surveillance role of T lymphocytes 41 might be promising. The gene discussed is CD274; the disease is intrahepatic cholangiocarcinoma.