Moreover, For the no BTS group, the multivariate regression analysis showed the risk factors for OS were CEA, CA19-9, γ-GGT, total bile acid, tumor number, tumor size, differentiation, lymph node metastasis (LNM), and for TTR were CEA, CA19-9, tumor number, tumor size, differentiation, LNM, macro-vessel invasion. This evidence concerns the gene TTR and neoplasm.