We also verify the expression of 11 hub genes in periodontitis patients and control samples by using qRT-PCR and IHC staining, the results showed that DLST, NLRP3 and GLS were highly expressed in periodontitis samples, while the expression of DLD, SLC31A1, DBT, NFE2L2, FDX1, LIAS and MTF1 were decreased in periodontitis samples.The expression of DLAT did not show any difference in periodontitis and control samples. Here, NLRP3 is linked to periodontitis.