Considering a large proportion of the above the 10 hub genes have been studied in the previous literature, such as IL-1B, MMP9, TLR2, TLR4, FOS, NR4A3, PTX3, which have a more definite association with cardiovascular disease, especially coronary heart disease (Abbate et al., 2020; Cheng et al., 2022; Hally et al., 2017; Martínez-González et al., 2021; Palomer et al., 2020; Ristagno et al., 2019; Zhu et al., 2022). This evidence concerns the gene MMP9 and cardiovascular disorder.