When individual variables were plotted in the ROC curve, a statistically significant correlation was established with WBC, Hb, Na, and creatinine, thus it can be concluded that patients with anemia (Hb < 10 g/dl), raised total white blood cell count (total WBC > 16,000 μL), raised CRP (>15 mg/dl), and hyponatremia (Na < 131 meq/L) harbor significant risk for progression to NF. The gene discussed is GSTM1; the disease is Hyponatremia.