NOTCH1 and acute lymphoblastic leukemia: To dissect the molecular mechanisms of RUNX1 and its relationship with NOTCH1, we performed histone ChIP-seq targeting six histone modifications (H3K4me1, H3K4me3, H3K9me3, H3K27me3, H3K36me3, and H3K27ac) in a T-ALL cell line (KOPTK1) following short hairpin RNA knockdown of RUNX1 (RUNX1-KD) or treatment with pharmacological inhibitor (Gamma secretase inhibitor [GSI]) of Notch signaling (NOTCH1-INB) (Figures 1A, S1A–S1C and Table S1).