CCND1 and hepatocellular carcinoma: Western blot assays uncovered that protein levels of three cell cycle-related proteins (CCNA2, CDK4, and CCND1) significantly decreased in HepG2 and Hep3B cell lines after knockdown PCNT expression (Figure 11A–11C), suggesting that PCNT might affect the progression of HCC through regulating the cell cycle signal.