Therefore, in C4-2B cells with higher PHB1 levels compared to LNCaP, FL3 reversed nucleus-cytoplasmic translocation trigged by ADT and consequently reversed the development of CRPC both in an AR-dependent and independent manner, which could explain why the C4-2B cells exhibited the highest sensitivity to FL3 among 4 tested PCa cell lines. Here, PHB1 is linked to posterior cortical atrophy.