Given that the C4-2B cell line with the highest sensitivity to FL3 is also extremely sensitive to ENZ [60], we then used MTS assay to compare the effect of FL3, ENZ, or FL3 combined with ENZ on the proliferation of AR+ PCa cell lines, LNCaP, C4-2B and 22Rv1, respectively. This evidence concerns the gene AR and posterior cortical atrophy.