MLC1 and type 2 diabetes mellitus: To investigate whether sAnk1.5 overexpression in skeletal muscle might result in predisposition to T2D, we subcloned the coding sequence of murine sAnk1.5 (GenBank accession number: U73972) into the pMEX expression vector, which carries the rat skeletal muscle MLC promoter and its enhancer and the SV40 poly-A sequence, thus generating the pMex-sAnk1.5 plasmid36.