CD4 and type 1 diabetes mellitus: For the analysis of T cells (Fig. 2A) we found several clusters that were larger among the T1D subjects, namely activated Tregs (cluster 1), activated CD4+ T cells (cluster 2), activated CD8+ T cells (cluster 3), CD4+CD8+ T cells (cluster 4), Th1 T cells (cluster 5), Th17 T cells that are the same cluster as the activated CD4+ T cells (cluster 2) and central memory CD8+ T cells that are the same cluster as the activated CD8+ T cells (cluster 3).