NDRG1 and hepatocellular carcinoma: The results showed that the proportion of dead SK-HEP1 and HCCLM3 cells showing red fluorescence significantly increased after NDRG1 knockdown, indicating that NDRG1 knockdown could significantly inhibit the survival of HCC cells (Fig. 7E, F).The EdU experiment results also showed that the EdU-positive rate of SK-HEP1 and HCCLM3 cells in the NDRG1 knockdown group was significantly lower than that in the control group, further confirming the inhibitory effect of NDRG1 knockdown on the proliferation of SK-HEP1 and HCCLM3 cells (p < 0.01) (Fig. 7G).