To confirm the role of VIRMA overexpression in breast cancer, we expressed the HA-tagged full-length VIRMA (VIRMA FL) and the VIRMA N-terminal isoform (VIRMA N-term) in breast cancer cell lines bearing low (SKBR3 and AU565), intermediate (MDA-MB-231) and high (HS578T) endogenous levels of VIRMA (Figs. 2A–B, S4A–C). This evidence concerns the gene VIRMA and breast carcinoma.