The major findings in the present study were (1) intraventricular injection of autologous blood caused ventricular dilatation, iron deposition, and a significantly upregulated expression of AQP4 in the periventricular tissue; (2) DFX inhibited hydrocephalus development and iron deposition as well as the upregulated expression of AQP4 in the periventricular tissue after IVH; and (3) TGN-020 attenuated ventricular dilatation and the expression of AQP4 following IVH without a significant effect on intraventricular iron deposition or ventricular wall damage. Here, AQP4 is linked to Hydrocephalus.