The evolution of NK cell subsets in CAR MLNK cells was similar to conventional CAR NK cells, but CD56bright CD16- population of CAR MLNK cells expressed a significant higher levels of IFN-γ than that of conventional CAR NK cells, resulting in enhanced selectively killing of CD19+ tumor cells including NK-resistant lymphoma cells, as compared with conventional CAR NK cells. The gene discussed is IFNG; the disease is lymphoma.