Furthermore, we want to investigate the correlation between WIPI2 and ferroptosis in colorectal cancer cells, the results of Western blot showed that WIPI2 knockdown (si-WIPI2) significantly inhibited the expression of ferroptosis protein ACSL4 and increased the expression of GPX4 compared with the NC-group (Figures 8A, B). Here, ACSL4 is linked to colorectal cancer.