For validation, IHC double stainings of PanCK/PD-L1, αSMA/PD-L1, and CD68/PD-L1 were performed showing that PD-L1 was more expressed by myofibroblasts and also macrophages rather than tumor cells (Supplementary Figures 2A-C). The gene discussed is ACTA1; the disease is neoplasm.