To further investigate the biological function of MINDY2 in PC, we discovered that the expression of MINDY2 was higher in PC cell lines (AsPC-1, BxPC-3, Capan-2, PANC-1, Mia PaCa-2, and SW1990) than in normal pancreatic ductal epithelial cells (HPDE); the lowest endogenous expression level of MINDY2 was seen in BxPC-3 cells and the highest expression in PANC-1 cells (Figure 4A). This evidence concerns the gene MINDY2 and pachyonychia congenita.