We further analyzed our single-cell sequencing data libraries to validate the changes of RANK+ subsets upon soyasaponin Bb treatment (Figure 7—figure supplement 1A), and we found that the percentage of monocytes was greater, while the transition to macrophage cells was lower, in the CIA mice treated with soyasaponin Bb mice relative to the vehicle treated mice (6.17% relative abundance of macrophages in the CIA treated group as compared to 24.3% in the untreated RA group) (Figure 7K). This evidence concerns the gene TNFRSF11A and rheumatoid arthritis.